Indentation tests are widely used to characterize the material properties of heterogeneous materials. So far there is no explicit analysis of the spatially distributed material properties for short fiber-reinforced composites on the mesoscale as well as a determination of the effective cross-section that is characterized by the obtained measurement results. Hence, the primary objective of this study is the characterization of short fiber-reinforced composites on the mesoscale. Furthermore, it is of interest to determine the corresponding area for which the obtained material parameters are valid. For the experimental investigation of local material properties of short fiber-reinforced composites, the Young’s modulus is obtained by indentation tests. The measured values of the Young’s modulus are compared to results gained by numerical simulation. The numerical model represents an actual microstructure derived from a micrograph of the used material. The analysis of the short fiber-reinforced material by indentation tests reveals the layered structure of the specimen induced by the injection molding process and the oriented material properties of the reinforced material are observed. In addition, the experimentally obtained values for Young’s modulus meet the results of a corresponding numerical analysis. Finally, it is shown, that the area characterized by the indentation test is 25 times larger than the actual projected area of the indentation tip. This leads to the conclusion that indentation tests are an appropriate tool to characterize short fiber-reinforced material on the mesoscale.