Fundamental studies of affinity separation of glycoproteins and its combination with expanded bed adsorption technique

Publication date
2006
Document type
PhD thesis (dissertation)
Author
Wen, Zhenzhen
Advisor
Niemeyer, Bernd
Granting institution
Helmut-Schmidt-Universität / Universität der Bundeswehr Hamburg
Exam date
2006-08-02
Organisational unit
DDC Class
600 Technik
Keyword
Affinitätstrennung
Expanded Bed Adsorption
Concanavalin A
Glucoseoxidase
Abstract
This work concerns the fundamental aspects of affinity separation of glycoconjugates, in particular, glycoproteins such as the enzyme glucose oxidase (GOD). It includes studies on the adsorption behaviour of GOD onto Concanavalin A (Con A) adsorbents, the preparation, characterization, and properties investigation of PEGylated Con A, the characterization of novel dense pellicular agarose coated glass beads and its application for expanded bed adsorption of proteins.
Diese Arbeit behandelt die fundamentalen Aspekte der Affinitätstrennung von Glykokonjugaten, insbesondere von Glykoproteinen am Beispiel des Enzym Glucose Oxidase (GOD). Die Studien beinhalten das Adsorptionsverhalten von GOD an Concanavalin A (ConA) Adsorbentien, deren Herstellung und Eigenschaften sowie Untersuchungen von PEGyliertem Con A und der Charakterisierung von einer neuen Matrix, die aus Agarose beschichteten Glasperlen (AG) besteht, und deren Einsatz in der Proteinadsorption mittels Expanded bed adsorption.
Version
Not applicable (or unknown)
Access right on openHSU
Open access